The promising guide to LC–MS analysis and cholinesterase activity of Luffa cylindrica (L.) fruit using in vitro and in-silico analyses

Abstract Background Luffa cylindrica (L.) is an annual climbing plant that produces fibrous fruit and can also be used as a vegetable in northern parts of India. Various studies have been carried out on the found to anti-inflammatory, antifungal, analgesic, anti-myocardial, anti-hyper triglyceride, immunostimulant, anti-allergic, other properties. The ethanolic extract has not yet subjected LC–MS analysis for several bioactive chemicals target neurological diseases. Oxidative stress inevitable situation AD mechanisms key bridge connecting various pathways. Results contains phytochemicals showed highest alkaloid content 21.39 ± 1.47 mg AE/g. A total 80 compounds were identified from analysis. screened eligibility by Lipinski's rule five docking with receptors responsible causing oxidative stress-associated Alzheimer's disease. Perlolyrine was chosen perform in-silico docking. An vitro activity cholinesterase inhibition at 500 µg/ml. In-silico perlolyrine better binding affinity score. revealed 10 docked receptors, amyloid beta energy − 46.1 kcal/mol showing promising drug Conclusion Based current findings, study reports presence promising, compound (perlolyrine) turn provides optimistic note exploring its biological vivo stress-related